Mass Spectrometry Unit
Equipment
Bi-dimentional liquid chromatography system coupled to an hibrid tandem mass spectrometer (triple quadropole – ion trap and QqTOF).

In what concerns analysis of small molecules, as peptides, the system acquires data useful for de novo sequencing and post-translational modifications identification, by combining the triple quadropole with the ion-trap. The fragmentation occurring in the collision cell out-side the ion trap allows access to information not provided by ion-trap fragmentation.

This system allows peptide and metabolite identification in the ion-trap mode, and further highly specific quantification in the triple quadropole mode using Multiple Reaction Monitoring (MRM). The system is also designed for detection, quantification and validation of biomarkers from biological samples.

http://www1.biocant.pt/MassSpectrometry_at_CNC
Services
-  Protein identification from digested samples
-  Metabolite Identification
-  Protein (from digested samples) and metabolite quantification (using calibration curves or specific quantification markers)
-  PTM’s Identification

http://www1.biocant.pt/MassSpectrometry_at_CNC/Services_and_RNEM.htm
Using the Facility
Samples have to be processed according to lab requirements (quantity and quality). Access to the equipment is restricted to authorized personal with specific knowledge on liquid chromatography, mass spectrometry, and associated software Due to the variety of possible hardware configurations, samples will be processed taking in consideration hardware configuration, date of arrival and specificity of analysis.
Unit fees
The price per samples depends on sample quality, intended analysis (both hardware and software) and report.
A aquisição destes equipamentos foi financiada através do QREN - Programa Operacional Regional do Centro 2007-2013 com o apoio do Mais Centro e da União Europeia no âmbito do projeto
"CNC Biotech - investigação em Biotecnologia e capacitação do sector empresarial"
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Bruno Manadas, PhD
Researcher and Unit Manager

Center for Neuroscience and Cell Biology University of Coimbra


Address:
UC-Biotech
Parque Tecnológico de Cantanhede,
Núcleo 04, Lote 8
3060-197 Cantanhede
PORTUGAL

Ph: +351 231 419 040

 
   
Financiado por Fundos FEDER através do Programa Operacional Factores de Competitividade – COMPETE 2020 e por Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia no âmbito do projecto Estratégico com referência atribuida pelo COMPETE: POCI-01-0145-FEDER-007440

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