Ines Miranda-Santos
351 936 717 360
Ph. D. Student
Group at CNC

2006 - 2016 - Ph. D. Biochemistry, University of Coimbra in collaboration with University of Saarland, Saarbrücken, Germany.
2002 - 2005 - M. Sci. Cell Biology, University of Coimbra, Portugal in collaboration with University of Texas, Southwestern, Dallas, Texas, U. S. A.
1997 - 2002 - Graduation (Licenciatura) Biochemistry, University of Coimbra, Portugal.
1979 - Birth, Coimbra, Portugal.

Area of Research: 

Intermediary metabolism of proliferating cells – why do proliferating cells present a metabolic phenotype so different of that of quiescent cells? The so called aerobic fermentation was first described by Warburg1and named after him as Warburg effect. Is this an impairment or an advantageous shift?After few decades of dormency, metabolism of proliferanting cells is now again under the limelight. A better understanding of it plays a pivotal role in the understanding of the physiology of differentiation of stem cells, development of embrionic cells and proliferation of tumour cells.

Fluxomics based in 13C metabolic flux analysis - Intermediary metabolic fluxes of proliferating cells can now be assessed as never before thanks methodologies based in 13C Metabolic Flux Analysis and computational models of metabolic networks. Cells are fed with labeled substrates selectively enriched with the tracer 13C. The 13C tracer is distributed through the metabolic network. The analysis and assessement of the trace distribution in the downstream metabolites allow tracing back the activity of the metabolic pathways, i.e. the metabolic fluxes.  

Modeling of metabolic networks - custom tailored metabolic network can now be implemented in available software suites2–5 which allow the calculation of metabolic fluxes.   The implementation of such custom tailored metabolic networks requires the reconstruction of the network of interest, the knowledge of the carbon mapping and of the thermodynamics of the reactions included in the reconstruction.

Research Summary:
Selected Publications: 

Miranda-Santos, I. et al.. Mass Isotopomer Analysis of Nucleosides Isolated from RNA and DNA Using GC/MS. Anal. Chem. 87, 617–23 (2015).


Other information: 

Research gate:
CNBC group webpage :
University of Saarland group webpage:


Financiado por Fundos FEDER através do Programa Operacional Factores de Competitividade – COMPETE 2020 e por Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia no âmbito do projecto Estratégico com referência atribuida pelo COMPETE: POCI-01-0145-FEDER-007440

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