Intermediary metabolism of proliferating cells – why do proliferating cells present a metabolic phenotype so different of that of quiescent cells? The so called aerobic fermentation was first described by Warburg1and named after him as Warburg effect. Is this an impairment or an advantageous shift?After few decades of dormency, metabolism of proliferanting cells is now again under the limelight. A better understanding of it plays a pivotal role in the understanding of the physiology of differentiation of stem cells, development of embrionic cells and proliferation of tumour cells.
Fluxomics based in 13C metabolic flux analysis - Intermediary metabolic fluxes of proliferating cells can now be assessed as never before thanks methodologies based in 13C Metabolic Flux Analysis and computational models of metabolic networks. Cells are fed with labeled substrates selectively enriched with the tracer 13C. The 13C tracer is distributed through the metabolic network. The analysis and assessement of the trace distribution in the downstream metabolites allow tracing back the activity of the metabolic pathways, i.e. the metabolic fluxes.
Modeling of metabolic networks - custom tailored metabolic network can now be implemented in available software suites2–5 which allow the calculation of metabolic fluxes. The implementation of such custom tailored metabolic networks requires the reconstruction of the network of interest, the knowledge of the carbon mapping and of the thermodynamics of the reactions included in the reconstruction.