| Microscopy Imaging Center of Coimbra - CNC (MICC - CNC) |
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| Centro de Microscopia e Imagiologia de Coimbra (MICC) |
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O Centro de Microscopia e Imagiologia de Coimbra constitui uma instalação centralizada onde os utilizadores recebem o suporte necessário para realizar técnicas convencionais e avançadas de imagiologia baseada em microscopia de luz. A unidade combina recursos para providenciar equipamento de ponta a todos os investigadores e empresas. O objetivo primário do MICC é melhorar o ambiente de investigação e de ensino para a comunidade científica CNC. Para atingir estas metas, o MICC:
 providencia treino técnico para utilizadores locais e investigadores visitantes;
oferece consultoria em desenho experimental e análise de imagem;
implementa avanços em hardware e software relevantes para as ciências biomédicas;
providencia educação continuada em abordagens avançadas de microscopia através de cursos e workshops.
O MICC é um nó do Portuguese Platform of BioImaging - PPBI, uma infrastructura do Roteiro Nacional de Infraestruturas de Relevância Estratégica, que recentemente se tornou parte do EuroBioImaging - ERIC e um parceiro da comunidade Zeiss labs@location.
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| MICC-CNC offers a wide range of different instruments for your research, from sample sectioning equipment to epifluorescence microscopy, high-speed confocal microscopy and laser catapulting microdissection, as well as other supporting equipment. The systems are prepared for advanced applications, including live cell imaging, and single-cell calcium measurement, enabling researchers to image dynamic events and molecular interactions. The P.A.L.M. laser microdissection microscope is a perfect tool for the isolation of the different cell population within a sample, allowing its full characterization. |
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How to access our resources
Before you access MICC resources you must read and sign the microscope usage police. This contains simple rules that you should follow when using MICC equipment.
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How to Book?
New users must schedule a hands-on training, with one of the MICC team member, before they can book any MICC equipment.
Go to the Agendo - Center for Neuroscience and Cell Biology web page: https://cnc.agendo.science/ and follow the How to book instructions.
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Publications
The mission of the MICC team is to guide and assist users in acquiring and analyzing images. Thus, it's not required to include any staff member of the imaging center in the author list of the publication. However, when a staff member of the imaging center has significantly contributed to a publication, it is highly recommended to add this person in the list of authors.
Mention of the imaging center in the publication: Users must indicate that they have used the MICC in any publication. For example, in the section Materials and Methods: "image acquisition [and image analysis] was done in the MICC Imaging facility of CNC" or in the acknowledgments section "The authors wish to thank [xxx] from the MICC Imaging facility of CNC”.
Users must submit, as soon as possible, the references of any publications that have used the equipment of the imaging center. These information highlight the use of the equipment of the imaging center, the efficiency of investment and facilitate all requests for funding for the acquisition of new microscopes.
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| Fees |
| Resource |
Price (€/hour) |
| CNC |
External Academic Units |
Commercial Use |
| Confocal LSM 710 |
11.00 * |
22.00 |
44.00 |
| Cell Observer Spinning-Disk |
10.00 * |
20.00 |
40.00 |
| AxioObserver Z1 |
NO fluorescence |
1.50 |
3.00 |
6.00 |
| Fluorescence |
2.00 |
4.00 |
8.00 |
| AxioImager Z2 |
NO fluorescence |
1.00 |
2.00 |
4.00 |
| Fluorescence |
1.50 |
3.00 |
6.00 |
| ApoTome2 (extra) |
1.00 |
2.00 |
4.00 |
| Axio Scan Z1 |
2.00 |
4.00 # |
4.00 # |
| Axioskop 2 Plus |
NO fluorescence |
0.50 |
1.00 |
2.00 |
| Fluorescence |
0.75 |
1.50 |
3.00 |
| Laser Microdissection (PALM) |
NO fluorescence |
0.75 |
1.50 |
3.00 |
| Fluorescence |
1.00 |
2.00 |
4.00 |
| Microdissection |
5.00 |
10.00 |
20.00 |
| Single Cell Calcium Imaging (SCCI) |
1.40 |
2.00 |
4.00 |
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* Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
# price upon request
▪ The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
▪ Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
▪ Reservations can be cancelled up to 12h prior to booked starting time.
▪ Unused reservations will be charged at full rate.
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| Microscopy Consumables |
| Consumables |
Unit Price (€) |
| IBIDI μ-Slide 4 well Untreated |
7.50 |
| IBIDI μ-Slide 8 well Untreated |
7.50 |
| IBIDI μ-Slide 8 well Treated |
7.50 |
| IBIDI μ-Slide 8 well Removable |
11.50 |
| IBIDI μ-Dish (35mm, high) glass bottom (5 dishes) |
16.00 |
| IBIDI μ-Dish (35mm, low; 5 dishes) |
14.50 |
| PALM MembraneSlide 1.0 PET (1 slide) |
6.00 |
| PALM AdhesiveCap 500 Clear (5 epp) |
20.00 |
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Quantitative Fluorescence Microscopy Course
5th – 9th October 2015
Center for Neuroscience and Cell Biology, polo I - UC
Read more here
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1st Hands-on Course on Fluorescence Microscopy and Image Analysis
22nd – 24th November 2013
Faculty of Health Sciences, University of Beira Interior, Covilhã
Read more here
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| Image Analysis Software: |
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| Online Tutorials and Books on Image Analysis: |
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Center for Neuroscience and Cell Biology
Faculty of Medicine of the University of Coimbra, Polo I, 2nd floor
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