| Microscopy Imaging Center of Coimbra - CNC (MICC - CNC) |
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| The Microscopy Imaging Center of Coimbra, at the Center for Neuroscience and Cell Biology (MICC-CNC), is a centralized facility where users receive the support required to carry out conventional and advanced imaging techniques, based on Light Microscopy. The unit has combined resources to provide state-of-the-art equipment that is open to all researchers. We offer the same services to academic investigators or companies. The primary goal of the MICC-CNC is to improve the research and teaching environment for the CNC scientific community. To meet these goals, the MICC-CNC: |
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provides technical training to local users and visiting researchers; |
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offers consultation on experimental design and image analysis; |
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implements advances in hardware and software relevant for biomedical sciences; |
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provides ongoing education in advanced microscopy approaches by organizing trainings courses and workshops. |
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| MICC-CNC is a node of the PPBI-Portuguese Platform of BioImaging, a infrastructure of National Roadmap of Research Infrastructures of Strategic Relevance. |
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| MICC-CNC offers a wide range of different instruments for your research, from epifluorescence microscopy, to high-speed confocal microscopy and laser catapulting microdissection. The systems are prepared for advanced applications, including live cell imaging, and single-cell calcium measurement, enabling researchers to image dynamic events and molecular interactions. The P.A.L.M. laser microdissection microscope is a perfect tool for the isolation of the different cell population within a sample, allowing its full characterization. |
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How to access our resources
First you should read the microscope usage policy, this contains simple rules that you should follow when using a microscope.
Then use our booking system in order to schedule some time on the system.
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Microscope Usage Policy
For new users, the first microscope session must be attended by the responsible technician. You should fill in the Ask for training form and send it to micc@cnc.uc.pt.
Before using a microscope users must have a valid reservation in the booking system.
Users must always use their own reserved time and should not use another user account to book time in a microscope. Use without a reservation is prohibited.
Booking changes must be made until 12h before the booking time.
Users should always use the Turn ON and Turn OFF hardware protocols.
The local hard disks are not for data storage. You need to remove your data. The disk might be wiped out without prior warning.
It is forbidden to use USB Flash drive, instead CD/DVD burning must be used.
Do not try to modify or install software on the microscope computers.
Only lab coats are allowed and gloves are not permitted to use on the microscopes. The microscopes are considered 'clean'.
If you find a problem with a microscope or evidence of incorrect usage, immediately contact the responsible technician.
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How to Book?
For new users, the first microscope session must be attended by the responsible technician. You should fill in the Ask for training form and send it to micc@cnc.uc.pt.
Go to the Agendo- Center for Neuroscience and Cell Biology web page: https://next.cirklo.org/cnc/ and follow the How to book instructions.
During working hours (8:00 to 20:00) users are allowed to book a maximum of 4 hours per day (12 hours per week) per system. Overnight (20:00 to 8:00) and weekend booking is not restricted.
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Publications
The mission of the MICC team is to guide and assist users in acquiring and analyzing images. Thus, it's not required to include any staff member of the imaging center in the author list of the publication. However, when a staff member of the imaging center has significantly contributed to a publication, it is highly recommended to add this person in the list of authors.
Mention of the imaging center in the publication: Users must indicate that they have used the MICC in any publication. For example, in the section Materials and Methods: "image acquisition [and image analysis] was done in the MICC Imaging facility of CNC" or in the acknowledgments section "The authors wish to thank [xxx] from the MICC Imaging facility of CNC”.
Users must submit, as soon as possible, the references of any publications that have used the equipment of the imaging center. These information highlight the use of the equipment of the imaging center, the efficiency of investment and facilitate all requests for funding for the acquisition of new microscopes.
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| Fees |
| Resource |
Price (€/hour) |
| CNC |
External Academic Units |
Commercial Use |
| Confocal LSM 710 |
11.00 * |
22.00 |
44.00 |
| Confocal LSM 510 Meta |
8.00 * |
16.00 |
32.00 |
| Spinning-Disk Cell Observer |
10.00 * |
20.00 |
40.00 |
| AxioObserver Z1 |
NO fluorescence |
1.50 |
3.00 |
6.00 |
| Fluorescence |
2.00 |
4.00 |
8.00 |
| AxioImager Z2 |
NO fluorescence |
1.00 |
2.00 |
4.00 |
| Fluorescence |
1.50 |
3.00 |
6.00 |
| ApoTome2 (extra) |
1.00 |
2.00 |
4.00 |
| Axioskop 2 Plus |
NO fluorescence |
0.50 |
1.00 |
2.00 |
| Fluorescence |
0.75 |
1.50 |
3.00 |
| Laser Microdissection (PALM) |
NO fluorescence |
0.75 |
1.50 |
3.00 |
| Fluorescence |
1.00 |
2.00 |
4.00 |
| Microdissection |
5.00 |
10.00 |
20.00 |
| Single Cell Calcium Imaging (SCCI) |
1.40 |
2.00 |
4.00 |
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* Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
▪ The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
▪ Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
▪ Reservations can be cancelled up to 12h prior to booked starting time.
▪ Unused reservations will be charged at full rate.
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| Microscopy Consumables |
| Consumables |
Unit Price (€) |
| IBIDI μ-Slide 8 well Untreated |
6.00 |
| IBIDI μ-Slide 8 well Treated |
6.00 |
| IBIDI μ-Dish (35mm, high) glass bottom (5 dishes) |
15.00 |
| PALM MembraneSlide 1.0 PET (1 slide) |
6.00 |
| PALM AdhesiveCap 500 Clear (5 epp) |
20.00 |
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Quantitative Fluorescence Microscopy Course
5th – 9th October 2015
Center for Neuroscience and Cell Biology, polo I - UC
Read more here
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1st Hands-on Course on Fluorescence Microscopy and Image Analysis
22nd – 24th November 2013
Faculty of Health Sciences, University of Beira Interior, Covilhã
Read more here
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Center for Neuroscience and Cell Biology
Faculty of Medicine of the University of Coimbra, Polo I, 2nd floor
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| Luísa Cortes, PhD
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| Unit Manager
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Ph: +351 239820190
Mobile: +351 932467382
Fax: + 351 239822776
Center for Neuroscience and Cell Biology
University of Coimbra
Rua Larga, Faculty of Medicine, Polo I, 1st floor
3004-504 Coimbra
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