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| Carl Zeiss LSM 510 META |
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Microscope overview
Carl Zeiss LSM 510 Meta is a laser scanning inverted confocal microscope equipped with multi-spectral detector, allowing spectral analysis. It has an excellent resolution, high power lasers and extended filter-sets.
The system is based on Axiovert 200M inverted microscope body and has live cell imaging facilities. Applications include, for example, time lapse imaging, photobleaching experiments, colocalization studies, spectral detection and 3D imaging.
LSM 510 Meta has two PMTs (photo multiplier tubes) and an adjustable META detector for collecting fluorescence emission from specimen: PMTs are highly sensitive and emission wavelengths are defined by default emission filters; META detector has multiply detector elements, instead, and can be used for spectral detection and subsequent linear unmixing of overlapping fluorescence stainings, however at a much lower sensitivity than its other two PMT detectors.
Furthermore, a transmission detector (PMT) detects the scanning laser and provides a transmission light image of the sample.
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| Charge Rates (€/hour – with IVA) |
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CNC Goups |
External Academic Groups |
Commercial Use |
| Self-Use |
8 |
16 |
32 |
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- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
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| LASER set |
| Laser Unit |
Wavelength |
Maximum Power |
| Diode 405-30 |
405 nm |
30 mW |
| Argon/2 |
458, 477, 488, 514 nm |
30 mW |
| DPSS 561-10 |
561 nm |
15 mW |
| HeNe633 |
633 nm |
5 mW |
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| Objectives |
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| Filter Sets (VIS Mode) |
| Filter Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 49 |
G 365 |
FT 395 |
BP 445/50 |
DAPI, Hoechst |
| Set 10 |
BP 470/40 |
FT 510 |
BP 540/50 |
Alexa 488, FITC, Calcein |
| Set 20 |
BP 546/12 |
FT 560 |
BP 607/65 |
Alexa 555, Rhodamine, PI |
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| Software |
| Image acquisition and analysis is performed using the LSM 510 software that includes the Physiology module. |
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