Carl Zeiss LSM 510 META
Microscope overview
Carl Zeiss LSM 510 Meta is a laser scanning inverted confocal microscope equipped with multi-spectral detector, allowing spectral analysis. It has an excellent resolution, high power lasers and extended filter-sets.
The system is based on Axiovert 200M inverted microscope body and has live cell imaging facilities. Applications include, for example, time lapse imaging, photobleaching experiments, colocalization studies, spectral detection and 3D imaging.
LSM 510 Meta has two PMTs (photo multiplier tubes) and an adjustable META detector for collecting fluorescence emission from specimen: PMTs are highly sensitive and emission wavelengths are defined by default emission filters; META detector has multiply detector elements, instead, and can be used for spectral detection and subsequent linear unmixing of overlapping fluorescence stainings, however at a much lower sensitivity than its other two PMT detectors.
Furthermore, a transmission detector (PMT) detects the scanning laser and provides a transmission light image of the sample.
Charge Rates (€/hour – with IVA)
  CNC Goups External Academic Groups Commercial Use
Self-Use 8 16 32
- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
LASER set
Laser Unit Wavelength Maximum Power
Diode 405-30 405 nm 30 mW
Argon/2 458, 477, 488, 514 nm 30 mW
DPSS 561-10 561 nm 15 mW
HeNe633 633 nm 5 mW
Objectives
Objective Magnification Numerical Aperture Medium Observation
EC-PlanNeofluar 10x 0,3 air Ph1, M27
Plan-ApoChromat 20x 0,8 air  
EC-PlanNeofluar 40x 1,3 oil DIC, M27
Plan-ApoChromat 63x 1,4 oil DIC, M27
Filter Sets (VIS Mode)
Filter Set Excitation Beam Splitter Emission Possible Dyes
Set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Set 10 BP 470/40 FT 510 BP 540/50 Alexa 488, FITC, Calcein
Set 20 BP 546/12 FT 560 BP 607/65 Alexa 555, Rhodamine, PI
Software
Image acquisition and analysis is performed using the LSM 510 software that includes the Physiology module.
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