Carl Zeiss LSM 710 (34 channels)
Microscope overview
The Carl Zeiss LSM 710 is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is based on Axio-Observer Z1 inverted microscope body, with motorized XY stage, and has live cell imaging facilities (stage insert for temperature and CO2 control). Applications include for example timelapse imaging, photobleaching experiments, colocalization studies, spectral detection and 3D imaging.
The LSM 710 scanning unit has a spectral recycling loop, and a QUASAR detection unit (34 PMTs). The QUASAR multichannel photomultiplier detection technology is based on a filter-free system that guides the desired wavelength range to the target detector using adjustable optical wedges and slider light stops. The QUASAR detector allow spectral scanning of fluorescence and emission “fingerprinting” as well as linear unmixing of signals from fluorophores with overlapping emission profiles.
Charge Rates (€/hour – with IVA)
  CNC Goups External Academic Groups Commercial Use
Self-Use 11 22 44
- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
LASER set
Laser Unit Wavelength Maximum Power
Diode 405-30 405 nm 30 mW
Argon/2 458, 488, 514 nm 25 mW
DPSS 561-10 561 nm 20 mW
HeNe633 633 nm 5 mW
Objectives
Objective Magnification Numerical Aperture Medium Observation
Plan-Apochromat 20x 0,8 air  
Plan-ApoChromat 40x 1,4 oil DIC, M27
Plan-Apochromat 63x 1,4 oil DIC, M27
Filter Sets (LOCATE Mode)
Filter Set Excitation Beam Splitter Emission Possible Dyes
Set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Set 38 (HE) BP 470/40 FT 495 BP 525/50 FITC, Alexa 488, GFP
Set 43 BP 545/25 FT 570 BP 605/70 Alexa 555, dTomato, PI
Software
Image acquisition and analysis is performed using the Zen Black 2012 software that includes the following modules:
-z Stack
-Time series
-Tiles and multi-position
-Autofocus
-Extended focus
-Physiology
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