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Microscope overview
The Carl Zeiss LSM 710 is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is based on Axio-Observer Z1 inverted microscope body, with motorized XY stage, and has live cell imaging facilities (stage insert for temperature and CO2 control). Applications include for example timelapse imaging, photobleaching experiments, colocalization studies, spectral detection and 3D imaging.
The LSM 710 scanning unit has a spectral recycling loop, and a QUASAR detection unit (34 PMTs). The QUASAR multichannel photomultiplier detection technology is based on a filter-free system that guides the desired wavelength range to the target detector using adjustable optical wedges and slider light stops. The QUASAR detector allow spectral scanning of fluorescence and emission “fingerprinting” as well as linear unmixing of signals from fluorophores with overlapping emission profiles.
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| Charge Rates (€/hour – with IVA) |
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CNC Goups |
External Academic Groups |
Commercial Use |
| Self-Use |
11 |
22 |
44 |
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- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
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| LASER set |
| Laser Unit |
Wavelength |
Maximum Power |
| Diode 405-30 |
405 nm |
30 mW |
| Argon/2 |
458, 488, 514 nm |
25 mW |
| DPSS 561-10 |
561 nm |
20 mW |
| HeNe633 |
633 nm |
5 mW |
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| Objectives |
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| Filter Sets (LOCATE Mode) |
| Filter Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 49 |
G 365 |
FT 395 |
BP 445/50 |
DAPI, Hoechst |
| Set 38 (HE) |
BP 470/40 |
FT 495 |
BP 525/50 |
FITC, Alexa 488, GFP |
| Set 43 |
BP 545/25 |
FT 570 |
BP 605/70 |
Alexa 555, dTomato, PI |
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| Software |
Image acquisition and analysis is performed using the Zen Black 2012 software that includes the following modules:
-z Stack
-Time series
-Tiles and multi-position
-Autofocus
-Extended focus
-Physiology
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