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Microscope overview
Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells.
Therefore, the Cell Observer Spinning Disk (SD) is a live cell imaging platform for high-speed confocal imaging using the CSU-X1M spinning disc technology from Yokogawa and one highly sensitive electron multiplying camera (EM-CCD Evolve Delta). This system is based on a motorized Carl Zeiss Axio Observer Z1 inverted scope with Definite Focus, multi-channel acquisition, piezo x/y/z stage, DirectFRAP unit, and a full environmental chamber for temperature and atmosphere control. The Definite Focus system allows for long-term time-lapse experiments without any focus drifts. In addition, the DirectFRAP is the ideal solution for any type of laser manipulation in the living cell – FRAP, FLIP, photoactivation or conversion.
Almost the entire microscope, from filter wheels to sample incubation parameters, are computer-controlled via the Zen Blue 2012 software.
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| Charge Rates (€/hour – with IVA) |
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CNC Goups |
External Academic Groups |
Commercial Use |
| Self-Use |
10 |
20 |
40 |
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- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
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| LASER set |
| Laser Unit |
Wavelength |
Maximum Power |
| Solid state laser 405 |
405 nm |
50 mW |
| Solid state laser 488 |
458 nm |
100 mW |
| Solid state laser 561 |
561 nm |
50 mW |
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| Objectives |
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| Filter Sets (LOCATE Mode) |
| Filter Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 49 |
G 365 |
FT 395 |
BP 445/50 |
DAPI, Hoechst |
| Set 38 (HE) |
BP 470/40 |
FT 495 |
BP 525/50 |
FITC, Alexa 488, GFP |
| Set 31 |
BP 565/30 |
FT 585 |
BP 620/60 |
Alexa 568, mCherry, mRFP |
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| Software |
Image acquisition and analysis is performed using the Zen Black 2012 software that includes the following modules:
-z Stack
-Time series
-Tiles and multi-position
-Autofocus
-Extended focus
-Physiology
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