Carl Zeiss Cell Observer Spinning Disk
Microscope overview
Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells.
Therefore, the Cell Observer Spinning Disk (SD) is a live cell imaging platform for high-speed confocal imaging using the CSU-X1M spinning disc technology from Yokogawa and one highly sensitive electron multiplying camera (EM-CCD Evolve Delta). This system is based on a motorized Carl Zeiss Axio Observer Z1 inverted scope with Definite Focus, multi-channel acquisition, piezo x/y/z stage, DirectFRAP unit, and a full environmental chamber for temperature and atmosphere control. The Definite Focus system allows for long-term time-lapse experiments without any focus drifts. In addition, the DirectFRAP is the ideal solution for any type of laser manipulation in the living cell – FRAP, FLIP, photoactivation or conversion.
Almost the entire microscope, from filter wheels to sample incubation parameters, are computer-controlled via the Zen Blue 2012 software.
Charge Rates (€/hour – with IVA)
  CNC Goups External Academic Groups Commercial Use
Self-Use 10 20 40
- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Extensive time laps studies (>4 hrs) are encouraged to be performed overnight (20:00 – 08:00) or at weekends at a reduced cost of 30%.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
LASER set
Laser Unit Wavelength Maximum Power
Solid state laser 405 405 nm 50 mW
Solid state laser 488 458 nm 100 mW
Solid state laser 561 561 nm 50 mW
Objectives
Objective Magnification Numerical Aperture Medium Observation
Plan-Apochromat 20x 0,8 air M27
LCI PlanNeofluar 63x 1,3 water or glycerol DIC, M27
Alpha Plan-Apochromat 100x 1,46 oil DIC, M27
Filter Sets (LOCATE Mode)
Filter Set Excitation Beam Splitter Emission Possible Dyes
Set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Set 38 (HE) BP 470/40 FT 495 BP 525/50 FITC, Alexa 488, GFP
Set 31 BP 565/30 FT 585 BP 620/60 Alexa 568, mCherry, mRFP
Software
Image acquisition and analysis is performed using the Zen Black 2012 software that includes the following modules:
-z Stack
-Time series
-Tiles and multi-position
-Autofocus
-Extended focus
-Physiology
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