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Microscope overview
The Axio Observer Z1 system is a fully motorized inverted widefield microscope, which is ideal for live imaging experiments. It is equipped with a large stage incubator for temperature and humidity control and a stage CO2 incubator.
The system is equipped with a CCD digital camera (Axiocam HRm) and a digital CMOS camera (ORCA Flash 4.0)
The ZEN Blue software allows to fully control the microscope and the cameras, allowing the acquisition of multidimensional images (multi-channel, time series, z-stack, tiles or multi-positions).
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| Charge Rates (€/hour – with IVA) |
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Illumination |
CNC Goups |
External Academic Groups |
Commercial Use |
| Self-Use |
Fluorescence |
2 |
4 |
8 |
| Transmission |
1,5 |
3 |
6 |
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- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
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| Filter Sets |
| Filter Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 49 |
G 365 |
FT 395 |
BP 445/50 |
DAPI, Hoechst |
| Set 38 (HE) |
BP 470/40 |
FT 495 |
BP 525/50 |
FITC, Alexa 488 |
| Set 31 |
BP 565/30 |
FT 585 |
BP 620/60 |
Alexa 568, mCherry |
| Set 50 |
BP 640/30 |
FT 660 |
BP 690/50 |
Alexa 667, Cy5 |
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| Objectives |
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| Software |
The acquisition and analysis of images is performed on the Zen Blue software 2012.
The software has the following modules:
- Multichannel
- Time series
- Tiles and multi-position
- z-Stack
- 3D VisArt
- Extended Focus
- Software Autofocus
- Experiment Manager
- Measurements
- Advance Processing
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