Carl Zeiss Axio Imager Z2
Microscope overview
The Axio Imager Z2 system is a fully motorized upright widefield microscope, which is ideal for quantitative analysis. Moreover, the system can perform structured illumination acquisition by means of ApoTome2.
The system is equipped with a CCD monochromatic digital camera (Axiocam HRm) and a CCD color digital camera (Axiocam HRc).
Stereological studies are also possible using StereoInvestigator software.
The ZEN Blue 2012 software fully controls the microscope and the cameras, allowing the acquisition of multidimensional images (multi-channel, time series, z-stack, tiles or multi-positions).
Charge Rates (€/hour – with IVA)
  Illumination CNC Goups External Academic Groups Commercial Use
Self-Use Fluorescence 1,5 3 6
Transmission 1 2 4
EXTRA ApoTome2 1 2 4
- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
Filter Sets
Filter Set Excitation Beam Splitter Emission Possible Dyes
Set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Set 38 (HE) BP 470/40 FT 495 BP 525/50 FITC, Alexa 488
Set 31 BP 565/30 FT 585 BP 620/60 Alexa 568, mCherry
Set 50 BP 640/30 FT 660 BP 690/50 Alexa 667, Cy5
Objective Magnification Numerical Aperture Medium Observation
EC Plan-Neofluar 5x 0,16 air  
Plan-Apochromat 20x 0,8 air  
Plan-Apochromat 40x 1,3 oil  
Plan-Apochromat 63x 1,4 oil DIC (motorized)
Software (the system contains two different software, which CANNOT be operated simultaneously):
The acquisition and analysis of images is performed on the Zen Blue software 2012.
The software has the following modules:
-z Stack
-Tiles and multi-position
-Experiment Designer

For stereology analysis, the system has the StereoInvestigator software.
The software has the following modules:
Financiado por Fundos FEDER através do Programa Operacional Factores de Competitividade – COMPETE 2020 e por Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia no âmbito do projecto Estratégico com referência atribuida pelo COMPETE: POCI-01-0145-FEDER-007440

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