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Microscope overview
Inverted widefield microscope designed for single cell calcium measurements. It is equipped with a Lambda-DG4 xenon lamp, allowing fast wavelength switching. Additionally, its sensitive cooled CCD camera (Roper Scientific CoolSnap EQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/ photodamage in light sensitive samples. The MetaFluor Imaging Software is designed for dual-wavelength intracellular ion measurements. The software allows simultaneous display of the raw data; ratio image; graphs of intensities, ratios, and ion concentration. Two different indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities.
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| Charge Rates (€/hour – with IVA) |
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CNC Goups |
External Academic Groups |
Commercial Use |
| Self-Use |
1,4 |
2 |
4 |
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- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
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| Filter Sets (Microscope) |
| Filter Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 10 |
BP 470/20 |
FT 510 |
BP 540/50 |
FITC, Alexa 488 |
| Set 15 |
BP 546/12 |
FT 580 |
LP 590 |
Rhodamina |
| Fura 2 |
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BS 400 |
BP 510/30 |
Fura 2 |
| Fura + Rhod |
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BS 430/60, 500/60, 570 |
BP 510/30 BP600/60 |
BP 510/30 BP600/60 Fura 2+TMRM |
| Set 02 |
G365 |
FT 395 |
LP 420 |
DAPI |
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| Filter Sets (Lambda-DG4 lamp house) |
| Filter Set |
Excitation |
Possible Dyes |
| 340 |
BP 340/20 |
Fura 2 |
| 380 |
BP 380/20 |
Fura 2 |
| 548 |
BP 548/20 |
TMRM |
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| Objectives |
| Objective |
Magnification |
Numerical Aperture |
Medium |
Observation |
| PlanNeofluar |
5x |
0,15 |
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Ph1 |
| PlanNeofluar |
20x |
0,5 |
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Ph2 |
| Fluar |
40x |
1,3 |
oil |
DIC |
| Fluar |
100x |
1,3 |
oil |
DIC |
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| Software |
| The acquisition and analysis of images is performed in the Metafluarn software. |
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