Zeiss/P.A.L.M. Laser Dissecting Microscope
Microscope overview
PALM MicroBeam employs the LMPC method (Laser Microdissection and Pressure Catapulting) to extract biological material of interest out of a tissue specimen. The Zeiss P.A.L.M. system employs a UV laser pulsed microbeam to cut the desired material and the isolated tissue is then recovered by laser pressure catapult into a microfuge tube cap. Cutting accuracy of less than 1 µm can be achieved.
The source material can be placed on a normal objective glass slide or on a membrane slide, depending on the way of approaching. The system can be used with frozen or paraffin-embedded tissue samples, single cells, and even single chromosomes.
Fluorescence optic is available for work with cells tagged with green fluorescent protein or other fluorescent markers.

For more information:

http://www.zeiss.com/microscopy/en_de/products/laser-microdissection/microbeam.html - webinars
Charge Rates (€/hour – with IVA)
  Illumination CNC Goups External Academic Groups Commercial Use
Self-Use Fluorescence 1 2 4
Transmission 0,75 1,5 3
Microdissection 5 10 20
- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
Filter Sets
Filter Set Excitation Beam Splitter Emission Possible Dyes
Set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Set 38 (HE) BP 470/40 FT 495 BP 525/50 FITC, Alexa 488, GFP
Set 43 BP 545/25 FT 570 BP 605/70 Alexa 555, dTomato, PI
Set 26 BP 600/50 FT 645 BP 685/50 Alexa 667, Cy5
Objective Magnification Numerical Aperture Medium Observation
Fluar 5x 0,25 air For microdissection
LD-PlanNeofluar 20x 0,4 air For microdissection;
Correction Ring (Coverglass thickness 0-1,5mm)
LD-PlanNeofluar 40x 0,6 air For microdissection;
Correction Ring (Coverglass thickness 0-1,5mm)
Plan-Apochromat 63x 1,4 oil DIC
PlanNeofluar 100x 1,3 oil  
Software (the system contains two different software, which CANNOT be operated simultaneously):
Axiovision Software (for Imaging Analysis)
   -z Stack
   -time lapse
   -Extended Focus
   -Automeasure Plus

PALM Robot Software (for Microdissection)
   -LMPC- Module
   -Extended Focus
   -Multichannel Fluorescence
Financiado por Fundos FEDER através do Programa Operacional Factores de Competitividade – COMPETE 2020 e por Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia no âmbito do projecto Estratégico com referência atribuida pelo COMPETE: POCI-01-0145-FEDER-007440

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