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Microscope overview
PALM MicroBeam employs the LMPC method (Laser Microdissection and Pressure Catapulting) to extract biological material of interest out of a tissue specimen. The Zeiss P.A.L.M. system employs a UV laser pulsed microbeam to cut the desired material and the isolated tissue is then recovered by laser pressure catapult into a microfuge tube cap. Cutting accuracy of less than 1 µm can be achieved.
The source material can be placed on a normal objective glass slide or on a membrane slide, depending on the way of approaching. The system can be used with frozen or paraffin-embedded tissue samples, single cells, and even single chromosomes.
Fluorescence optic is available for work with cells tagged with green fluorescent protein or other fluorescent markers.
For more information:
http://microscopy.zeiss.com/microscopy/en_de/products/laser-microdissection/microbeam.html
Webinars:
http://www.zeiss.com/microscopy/en_de/products/laser-microdissection/microbeam.html - webinars
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| Charge Rates (€/hour – with IVA) |
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Illumination |
CNC Goups |
External Academic Groups |
Commercial Use |
| Self-Use |
Fluorescence |
1 |
2 |
4 |
| Transmission |
0,75 |
1,5 |
3 |
| Microdissection |
5 |
10 |
20 |
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- The charge for CNC users corresponds to consumables and 50% of service contracts / parts replacement.
- Imaging acquisition by MICC technician will be charged 3€ per hour (excluding training and technical support).
- Reservations can be cancelled up to 12h prior to booked starting time.
- Unused reservations will be charged at full rate.
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| Filter Sets |
| Filter Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 49 |
G 365 |
FT 395 |
BP 445/50 |
DAPI, Hoechst |
| Set 38 (HE) |
BP 470/40 |
FT 495 |
BP 525/50 |
FITC, Alexa 488, GFP |
| Set 43 |
BP 545/25 |
FT 570 |
BP 605/70 |
Alexa 555, dTomato, PI |
| Set 26 |
BP 600/50 |
FT 645 |
BP 685/50 |
Alexa 667, Cy5 |
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| Objectives |
| Objective |
Magnification |
Numerical Aperture |
Medium |
Observation |
| Fluar |
5x |
0,25 |
air |
For microdissection |
| LD-PlanNeofluar |
20x |
0,4 |
air |
For microdissection;
Correction Ring (Coverglass thickness 0-1,5mm) |
| LD-PlanNeofluar |
40x |
0,6 |
air |
For microdissection;
Correction Ring (Coverglass thickness 0-1,5mm) |
| Plan-Apochromat |
63x |
1,4 |
oil |
DIC |
| PlanNeofluar |
100x |
1,3 |
oil |
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| Software (the system contains two different software, which CANNOT be operated simultaneously): |
Axiovision Software (for Imaging Analysis)
-Modules:
-Multichannel
-z Stack
-mosaiX
-time lapse
-Extended Focus
-Automeasure Plus
-Commander
PALM Robot Software (for Microdissection)
-Modules:
-Autofocus
-LMPC- Module
-Extended Focus
-Multichannel Fluorescence
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