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| Research in brain cancer: genetic heterogeneity of gliomas |
Alberto Orfao (Centro de Investigación del Cancer, Salamanca), Fernando Gomes (HUC),
Celeste Lopes (CNC) |
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| The project ‘Assessment of intratumoral genetic heterogeneity in gliomas: impact on the clinical and biological behaviour of the disease’ is being developed in collaboration with the Neuropathology Laboratory and Neurosurgery Service of University Hospital of Coimbra and with the Research Unit of the University Hospital and Center for Cancer Research of Salamanca. In this project, allelic imbalances in chromosome regions of human gliomas are evaluated using interphase fluorescence in situ hybridization (iFISH) and the gene expression profiling is now starting to be performed by cDNA micro-arrays and SNP-array analysis. Tissue samples are obtained from patients diagnosed with gliomas, undergoing surgery at the Neurosurgical Unit of the University Hospital of Coimbra. The iFISH evaluation of allelic imbalances detected numerical abnormalities in the following chromosome regions: 1p36, 19q13, 7q11, 9p21, 9q34, 10q23, 13q14, 17p13 and 22q11, confirming the existence of complex cytogenetic abnormalities in these tumours. The analysis of larger groups of patients will help to establish the potential clinical relevance of these findings. |
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| PUBLICATIONS |
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| Sayagues JM, Tabernero MD, Maillo A, Trelles O, Espinosa AB, Sarasquete ME, Merino M, Rasillo A, Vera JF, Santos-Briz A, de Alava E, Garcia-Macias MC, Orfao A. (2006). Microarray-based analysis of spinal versus intracranial meningiomas: different clinical, biological, and genetic characteristics associated with distinct patterns of gene expression. J Neuropathol Exp Neurol, 65:445-54. |
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| Espinosa AB, Tabernero MD, Maillo A, Sayagues JM, Ciudad J, Merino M, Alguero MC, Lubombo AM, Sousa P, Santos-Briz A, Orfao A. (2006). The cytogenetic relationship between primary and recurrent meningiomas points to the need for new treatment strategies in cases at high risk of relapse. Clin Cancer Res.;12:772-80. |
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