Almeida C.M., Simões I. Cardoon-based rennets for cheese production Appl Microbiol Biotechnol (2018) https://doi.org/10.1007/s00253-018-9032-3
Almeida, C.M., Manso, J.A., Figueiredo, A.C. et al. (2017) Functional and structural characterization of synthetic cardosin B-derived rennet Appl Microbiol Biotechnol. 101: 6951. https://doi.org/10.1007/s00253-017-8445-8
Alves, A., Caeiro, A., Correia, S.I. et al. (2017) Establishment and biochemical characterization of tamarillo (Solanum betaceum Cav.) embryogenic cell suspension cultures In Vitro Cell.Dev.Biol.-Plant. https://doi.org/10.1007/s11627-017-9864-z
Curto P., Simões I., Riley S.P., Martinez J.J. (2016) Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells. Front. Cell. Infect. Microbiol. 6:80. DOI: 10.3389/fcimb.2016.00080.
Leal AR, Cruz R, Bur D, Huesgen PF, Faro R, Manadas B, Wlodawer A, Faro C, Simões I. (2016) Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans. Sci Rep. Mar 31;6:23869. doi: 10.1038/srep23869.
Dias DM, Furtado J, Wasielewski E, Cruz R, Costello B, Cole L, Faria TQ, Baaske P, Brito RM, Ciulli A, Simões I, Macedo-Ribeiro S, Faro C, Geraldes CF, Castanheira P. (2016) Biophysical characterization of laforin-carbohydrate interaction. Biochem J. Feb 1;473(3):335-45. doi: 10.1042/BJ20141555. Epub 2015 Nov 17.
Gustchina, Mi Li, R. Cruz, M. Simões, P. Curto, J. Martinez, C. Faro, I. Simões and A. Wlodawer (2016) Crystal structure of the soluble domain of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease Acta Cryst. A72, s217
Li M, Gustchina A, Cruz R, Simões M, Curto P, Martinez J, Faro C, Simões I, Wlodawer A. (2015) Structure of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease. Acta Crystallogr D Biol Crystallogr. Oct;71(Pt 10):2109-18. doi: 10.1107/S1399004715013905.
Almeida CM, Gomes D, Faro C, Simões I. (2015) Engineering a cardosin B-derived rennet for sheep and goat cheese manufacture. Appl Microbiol Biotechnol. Jan;99(1):269-81. doi: 10.1007/s00253-014-5902-5.
Faria TQ, Almeida ZL, Cruz PF, Jesus CS, Castanheira P, Brito RM. (2015) A look into amyloid formation by transthyretin: aggregation pathway and a novel kinetic model. Phys Chem Chem Phys. Mar 21;17(11):7255-63. doi: 10.1039/c4cp04549a.
Fernandes JP, Neto R, Centeno F, Castanheira P, De Fátima Teixeira M, Gomes AC. (2015) Unveiling the potential of novel yeast protein extracts in white wines clarification and stabilization. Front Chem. May 8;3:31. doi: 10.3389/fchem.2015.00031. eCollection 2015.
Cruz R, Huesgen P, Riley SP, Wlodawer A, Faro C, Overall CM, Martinez JJ, Simões I. (2014) RC1339/APRc from Rickettsia conorii is a novel aspartic protease with properties of retropepsin-like enzymes. PLoS Pathog. Aug 21;10(8):e1004324. doi: 10.1371/journal.ppat.1004324. eCollection 2014 Aug.
Curto P, Lufrano D, Pinto C, Custódio V, Gomes AC, Trejo SA, Bakás L, Vairo-Cavalli S, Faro C, Simões I. (2014) Establishing the yeast Kluyveromyces lactis as an expression host for production of the saposin-like domain of the aspartic protease cirsin. Appl Environ Microbiol. Jan;80(1):86-96. doi: 10.1128/AEM.03151-13.
Videira, R; Castanheira, P; Grãos, M; Resende, R; Salgueiro, L; Cavaleiro, C and Faro, C. (2014) “Dose-Dependent Inhibition of BACE-1 by the Monoterpenoid 2,3,4,4-Tetramethyl-5-methylene-cyclopent-2-enone in Cellular and Mouse Models of Alzheimer’s Disease” Journal of Natural Products 77(6):1275-1279.
Azul, AM ; Nunes, J ; Ferreira, I ; Coelho, AS ;Verissimo, P ; Trovao, J ; Campos, A ; Castro, P ; Freitas, H (2014) “Valuing native ectomycorrhizal fungi as a Mediterranean forestry component for sustainable and innovative solutions” Botany, 2014, 92(2): 161-171, https://doi.org/10.1139/cjb-2013-0170
Resende R, Sueli C., Ferreiro BE., Simoes I., Oliveira C., Pereira, C. (2013) Effect of a-synuclein on amyloid ß-induced toxicity. Relevance to Lewy Body Variant of Alzheimer disease. Neurochemical Research DOI 10.1007/s11064-013-0982-7
Videira, R., Castanheira, P., Grãos, M., Salgueiro, L., Faro, C. and Cavaleiro, C. (2013), A necrodane monoterpenoid from Lavandula luisieri essential oil as a cell-permeable inhibitor of BACE-1, the β-secretase in Alzheimer's disease. Flavour Fragr. J., 28: 380–388. doi:10.1002/ffj.3156
Paiva G, Proença DN, Francisco R, Verissimo P, Santos SS, Fonseca L, Abrantes IM, Morais PV Nematicidal bacteria associated to pinewood nematode produce extracellular proteases. PLoS One. 2013 Nov 7;8(11):e79705. doi: 10.1371/journal.pone.0079705. eCollection 2013.
Almeida, C., Simões, I., Faro C., US Patent No. 9,890,372, granted 13 February 2018 – Aspartic Proteases: Methods and composition, thereof, relate to aspartic proteases, and particularly to aspartic proteases for plants. Disclosed are modified plant aspartic proteases, and methods for their manufacture, and uses thereof. Particularly contemplated are the uses of aspartic proteases in inducing skin desquamation.
Almeida, C., Simões, I., Faro C., Patent Application PCT/PT2014/000018; Publication number WO2014148932 A3 – Use of plant aspartic proteases for treating skin conditions, 2014. Applicant: Biocant - Associação De Transferência De Tecnologia, Cantanhede, Portugal.
Almeida, C., Simões, I., Faro C., Patent Application PCT/PT2014/000017; Publication number WO2014148931 A3 – Plant aspartic proteases with deleted psi motifs, 2014. Applicant: Biocant - Associação De Transferência De Tecnologia, Cantanhede, Portugal.
2010 to present - Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
Auxiliary Investigator, equivalent to Assistant Professor
Oct 2015 to Dec 2016 - Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, USA
Visiting Assistant Professor, sabbatical leave in the Vector-borne Disease Laboratories at the School of Veterinary Medicine
2011 to 2014 - Cynara Lda., (Biocant spin-off)
Co-founder/ Manager, spin-off focused on commercializing synthetic rennet for cheese production (patent application)
2008/2009 - Department of Biochemistry, University of Coimbra
Invited Assistant Professor, Responsible for two core disciplines: Molecular Biology (lecture) and Laboratories of Molecular Biology (practical)
Sep 2007 to Dec 2007 - University of Massachusetts , Amherst, MA Visiting Researcher, with Dr. Alice Y. Cheung
Jan 2006 to Sep 2010 - Biocant - Biotechnology Innovation Center, Cantanhede, Portugal
Researcher, participated in the establishment of the research Unit of Molecular Biotechnology
2004 to 2005 - Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
Post-Doctoral Researcher, with Dr. Carlos Faro
2004 - PhD Biochemistry (Enzymology), University of Coimbra, Portugal
Advisor: Dr. Euclides Pires (University of Coimbra) and Dr. Alice Y. Cheung (University of Massachusetts, Amherst, USA)
1998 - M.Sc. Cell Biology, University of Coimbra, Portugal
Faculty of Sciences and Technology, Advisor: Dr. Euclides Pires
1996 - B.Sc. Biochemistry, University of Coimbra, Portugal
Department of Biochemistry, Faculty of Sciences and Technology
Fellowships and Awards
1998 PhD Fellowship, Portuguese Foundation for Science and Technology
2003 Travel Award to participate in the 3rd General Meeting of the International Proteolysis Society, Nagoya, Japan
2004 Post-doctoral Fellowship, Portuguese Foundation for Science and Technology
2004 Honorary Mention - Young Entrepreneur Award, 5th Edition, Academia dos Empreendedores - Associação Nacional de Jovens Empresários (ANJE), Portugal
2004 European Regional Innovation Awards (X-PROT Project) – 2nd Place (Team Member) European Union - Committee of the Regions
2005 Travel award for the participation in 4th General Meeting of the International Proteolysis Society, Quebec, Canada
2017 Travel Award for participation: "ESCCAR 2017 - International congress on Rickettsia and other intracellular bacteria", American Society for Rickettsiology, Marseille, France
2017 Bio Enterpreneur Talent Award – A synthetic cardosin B-derived rennet for sheep and goat cheese manufacture, MICROBIOTEC17 Congress of Microbiology and Biotechnology 17, 7-9 December, Porto, Portugal