| Contact |
Bruno Manadas
Center for Neuroscience and Cell Biology
University of Coimbra
3004-517 Coimbra
Portugal
Ph. +351 91 351 03 46
bmanadas@gmail.com
http://cnc.cj.uc.pt/~proteome/
http://cnc.cj.uc.pt/~cbduarte/
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| Location |
| Biocant Campus, Advanced Services Unit |
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| Equipment |
Bi-dimentional liquid chromatography system coupled to an hibrid tandem mass spectrometer (triple quadropole – ion trap).
The 4000 QTRAP is a unique system coupling the triple quadropole specificity with ion-trap sensitivity.
In what concerns analysis of small molecules, as peptides, the system acquires data useful for de novo sequencing and post-translational modifications identification, by combining the triple quadropole with the ion-trap. The fragmentation occurring in the collision cell out-side the ion trap allows access to information not provided by ion-trap fragmentation.
This system allows peptide and metabolite identification in the ion-trap mode, and further highly specific quantification in the triple quadropole mode using Multiple Reaction Monitoring (MRM). The system is also designed for detection, quantification and validation of biomarkers from biological samples.
http://cnc.cj.uc.pt/~proteome/
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| Services |
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Protein identification from digested samples |
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Metabolite Identification |
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Protein (from digested samples) and metabolite quantification (using calibration curves or specific quantification markers) |
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PTM’s Identification |
http://cnc.cj.uc.pt/~proteome/Services_and_RNEM.htm/ |
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| Using the Facility |
| Samples have to processed according to lab requirements (quantity and quality). Access to the equipment is restricted to authorized personal with specific knowledge on liquid chromatography, mass spectrometry, and associated software (Ultimate 3000 - LCPackings; 4000 QTRAP MSMS – Applied Biosystems; Chromeleon – Dionex; Analyst – Applied Biosystems). Due to the variety of possible hardware configurations, samples will be processed taking in consideration hardware configuration, date of arrival and specificity of analysis. |
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| Unit fees |
| The price per samples depends on sample quality, intended analysis (both hardware and software) and report. |
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Este equipamento foi financiado ao abrigo do Programa Nacional de Reequipamento Científico através da Fundação para a Ciência e a Tecnologia.
- Programa Nacional de Re-equipamento Científico e do Programa que co-financiou (POCI 2010, FEDER)
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Governo da República Portuguesa |
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União Europeia — Fundos Estruturais |
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