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Microscope overview A laser scanning confocal microscope with spectral analysis. It has an excellent resolution, high power lasers and extended filter. |
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| LASER sets |
| Laser Unit |
Wavelength |
Maximum Power |
| Diode 405-30 |
405 nm |
30 mW |
| Argon/2 |
458, 477, 488, 514 nm |
45 mW |
| DPSS 561-10 |
561 nm |
15 mW |
| HeNe633 |
633 nm |
5 mW |
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| Objectives |
| Objective |
Magnification |
Numerical Aperture |
Medium |
Observation |
| EC-PlanNeofluar |
10x |
0,3 |
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Ph1 |
| Plan-ApoChromat |
20x |
0,8 |
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| EC-PlanNeofluar |
40x |
1,3 |
oil |
DIC |
| Plan-ApoChromat |
63x |
1,4 |
oil |
DIC |
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| Software |
| The acquisition and analysis of images is performed in the LSM 510 software. |
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| Microscope Turn On Procedures |
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Turn on the two main switches: 1st System/PC and 2nd Equipments |
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Login to the computer, start the LSM510 software. |
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| Microscope Turn Off procedures |
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| If there's another user for this microscope in the next two hours: |
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Close the software, leave the lasers on. |
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Clean up any objective were you used immersion oil. |
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Make sure that there really is another user going to use the microscope. |
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| Else: |
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Clean up any objective were you used immersion oil. |
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Turn off the lasers in the software and shut down the computer. |
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Wait 5 minutes for laser cooldown. |
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Turn off the two main switches. |
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