Microscopy
Zeiss LSM510 META
Microscope overview
A laser scanning confocal microscope with spectral analysis. It has an excellent resolution, high power lasers and extended filter.
LASER sets
Laser Unit Wavelength Maximum Power
Diode 405-30 405 nm 30 mW
Argon/2 458, 477, 488, 514 nm 45 mW
DPSS 561-10 561 nm 15 mW
HeNe633 633 nm 5 mW
Objectives
Objective Magnification Numerical Aperture Medium Observation
EC-PlanNeofluar 10x 0,3   Ph1
Plan-ApoChromat 20x 0,8    
EC-PlanNeofluar 40x 1,3 oil DIC
Plan-ApoChromat 63x 1,4 oil DIC
Software
The acquisition and analysis of images is performed in the LSM 510 software.
Microscope Turn On Procedures
Turn on the two main switches: 1st System/PC and 2nd Equipments
Login to the computer, start the LSM510 software.
Microscope Turn Off procedures
If there's another user for this microscope in the next two hours:
Close the software, leave the lasers on.
Clean up any objective were you used immersion oil.
Make sure that there really is another user going to use the microscope.
Else:
Clean up any objective were you used immersion oil.
Turn off the lasers in the software and shut down the computer.
Wait 5 minutes for laser cooldown.
Turn off the two main switches.
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