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Microscope overview
PALM MicroBeam employs the LMPC method (Laser Microdissection and Pressure Catapulting). Laser capture microdissection microscopy (LCMM) utilizes a UV laser pulsed microbeam for dissection. The isolated tissue is then recovered by laser pressure catapult into a microfuge tube cap. The UV wavelength of the laser is too long to damage polynucleotides. LCMM is suitable for most routinely prepared cell and tissues samples, including single cells and microbial applications. The Zeiss/P.A.L.M. system can also be used with live tissues and for subcellular dissection. Fluorescence optics is available for work with cells tagged with green fluorescent protein or other fluorescent markers. |
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For more information: http://www.palm-microlaser.com
Demo movies: http://www.palm-microlaser.com/dasat/index.php
The system is coupled to a Axiovert 200M microscope.
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| Filters sets |
| Filters Set |
Excitation |
Beam Splitter |
Emission |
Possible Dyes |
| Set 49 |
G 365 |
FT 395 |
BP 445/50 |
DAPI, Hoechst |
| Set 38 (HE) |
BP 470/40 |
FT 495 |
BP 525/50 |
FITC, Alexa 488 |
| Set 31 |
BP 565/30 |
FT 585 |
BP 620/60 |
Alexa 568, mCherry |
| Set 50 |
BP 640/30 |
FT 660 |
BP 690/50 |
Alexa 660, Cy5 |
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| Objectives |
| Objective |
Magnification |
Numerical Aperture |
Medium |
Observation |
| Fluar |
5x |
0,25 |
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| LD-PlanNeofluar |
20x |
0,4 Korr |
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For microdissection |
| LD-PlanNeofluar |
40x |
0,6 Korr |
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For microdissection |
| Plan-ApoChromat |
63x |
1,4 |
oil |
DIC |
| Plan-ApoChromat |
40x |
1,3 |
oil |
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| Software |
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| The system contains two different softwares: |
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Axiovision Software (for Imaging analysis) |
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PALM RoboSoftware (for Microdissection) |
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| The two softwares can NOT be operated simultaneously. |
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